JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
RhoC的GTP酶激活法
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
RhoC GTPase Activation Assay
Please note that all translations are automatically generated.
Click here for the English version.
RhoC的GTP酶激活法
DOI:
10.3791/2083-v
•
09:58 min
•
August 22, 2010
•
Michelle Lucey
,
Heather Unger
,
Kenneth L. van Golen
1
Department of Biological Sciences, The Center for Translational Cancer Research
,
University of Delaware
Chapters
00:00
Title
00:48
Introduction
01:25
Preparation of GST-fusion Protein
04:41
GST-RBD Pull Down Assay
09:01
Representative Results for a RhoC GTPase Activation Assay
09:34
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议利用上拉下来检测,以确定细胞内的活动中RhoC GTP酶的水平。
Tags
RhoC GTPase
RhoA GTPase
Activation Assay
Homology
Reagents
Techniques
Metastatic Cancer Cells
Rho Activation Assay
GST-Rho Binding Domain Fusion Protein
Active RhoC GTPase
Total Protein
GTP Bound
GDP Bound
Commercial Versions
Commercial Kits
RhoC-specific Antibody
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
专访:糖脂抗原提呈CD1d及NKT细胞活化治疗潜力
自由表现动物的神经回路活化的录音
主动脉环含量
分离和<em在体外激活
线虫
精子
激活细胞凋亡的细胞色素的细胞质显微注射
C</em
细胞内复性检测
酰胺氢/氘交换和MALDI - TOF质谱PAK2激活的质谱分析
单分子成像的基因调控<em在体内</em使用转位激活裂解(CoTrAC)
线虫
趋含量的
比较GTP酶结合蛋白的使用竞争性试验的亲和
Read Article