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Journal
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Immunology and Infection
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白细胞的归位和迁移的非侵入性的成像
在体内</em
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Non-invasive Imaging of Leukocyte Homing and Migration
in vivo
Please note that all translations are automatically generated.
Click here for the English version.
白细胞的归位和迁移的非侵入性的成像
在体内</em
DOI:
10.3791/2062-v
•
07:42 min
•
December 05, 2010
•
Baomei Wang
,
Bernd H. Zinselmeyer
2
,
Jeremiah R. McDole
,
Peggy A. Gieselman
,
Mark J. Miller
1
Department of Pathology and Immunology
,
Washington University in St. Louis
,
2
National Institute of Neurological Disorders and Stroke, NINDS
,
NIH – National Institute of Health
Chapters
00:05
Title
00:55
Introduction
01:25
Animal Preparation
02:19
Delivering an Inflammatory Stimulus to Induce Leukocyte Recruitment
03:19
Imaging Stage Setup
04:17
Video Acquisition
06:55
Representative Time-Lapse Images
07:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一种非侵入性的双光子显微镜(2P)的方法来研究白细胞在鼠标脚垫的归巢。我们将讨论我们的组织成像中的技术方面的准备,然后步行通过设立执行和数据收集的实验,从最初的典型读者。
Tags
Non-invasive Imaging
Leukocyte Homing
Leukocyte Migration
In Vivo Imaging
Two-photon Microscopy
High Resolution Imaging
Cell Behavior
Live Mice
Leukocyte Recruitment
Host Defense
Infection
Inflammatory Diseases
Autoimmune Diseases
Intravital Imaging
Surgical Preparation
Blood Vessels
Inflammation
Non-invasive Single-cell Imaging
Leukocyte Trafficking
Mouse Footpad And Phalanges
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