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Journal
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Immunology and Infection
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在固定和活细胞,流式细胞仪测量钙蛋白酶活性
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
在固定和活细胞,流式细胞仪测量钙蛋白酶活性
DOI:
10.3791/2050-v
•
11:37 min
•
July 08, 2010
•
Christina Farr
,
Stuart Berger
2
1
Immunology
,
University of Toronto
,
2
Arthritis and Immune Disorder Research Centre
,
University Health Network (UHN)
Chapters
00:06
Title
00:48
Introduction
01:15
Reagent Preparation
01:56
Cell Preparation
03:11
Calpain Assay in Fixed Cells
04:48
Data Acquisition (Fixed Cells)
06:55
Calpain Assay in Live Cells
08:22
Calpain Assay in Mixed Cells
10:05
Analysis and Representative Results
11:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文将详细测量固定和生活用流式细胞仪检测细胞中的蛋白酶活动的协议。
Tags
Calpain Activity
Flow Cytometry
Fixed Cells
Living Cells
Calcium-sensitive Proteases
Physiological Processes
Signaling
Cytoskeletal Remodeling
Gene Expression Regulation
Apoptosis
Cell Cycle Progression
Pathologies
Muscular Dystrophies
Cancer
Diabetes
Alzheimer’s Disease
Multiple Sclerosis
Calpain Regulation
MRNA Levels
Protein Levels
Gene Expression Techniques
Fluorescent Substrate BOC-LM-CMAC
Calpain Activity Measurement
Murine 32Dkit Leukemia Cells
Splenocyte Population
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