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一个反向遗传学方法测试胚胎发育过程中的功能冗余
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Biology
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Biology
A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis
Please note that all translations are automatically generated.
Click here for the English version.
一个反向遗传学方法测试胚胎发育过程中的功能冗余
DOI:
10.3791/2020-v
•
06:59 min
•
August 11, 2010
•
Amir Rikin
,
Gabriel E. Rosenfeld
,
Kellie McCartin
,
Todd Evans
1
Department of Surgery
,
Weill Cornell Medical College of Cornell University
Chapters
00:07
Title
00:59
Introduction
01:55
Validation of Morpholinos
04:23
Co-Injection of Morpholinos
04:54
Evaluation of Phenotypes
05:37
Representative Reuslts
06:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
基因的功能,可以掩盖损失功能的实验中,如果有另一种基因的赔偿。斑马鱼模型提供了一个相对高通量的手段来揭示生活胚胎这样的功能冗余。
Tags
Reverse Genetic Approach
Functional Redundancy
Embryogenesis
Loss-of-function Experiments
Targeted Mutagenesis
Knockout
Zebrafish Model
Gene-specific Antisense Morpholinos
MRNA
Translation Inhibition
Splicing Inhibition
Knockdown
Gene Compensation
Gene Families
Sister Genes
Co-expression
High-throughput Testing
Genetic Interaction
Sub-threshold Levels
GATA Transcription Factor
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