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Genetics
/
A High-Throughput UAS-cDNA/ORF Plasmid Library Construction Using CRISPRmass
/
Large-Scale Construction of UAS-cDNA/ORF Plasmids by CRISPR-Based Modular Assembly (CRISPRmass)
JoVE Journal
Genetics
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JoVE Journal
Genetics
Large-Scale Construction of UAS-cDNA/ORF Plasmids by CRISPR-Based Modular Assembly (CRISPRmass)
Large-Scale Construction of UAS-cDNA/ORF Plasmids by CRISPR-Based Modular Assembly (CRISPRmass)
A High-Throughput UAS-cDNA/ORF Plasmid Library Construction Using CRISPRmass
DOI:
10.3791/201691-v
•
02:25 min
•
May 17, 2024
•
Si Xu*
1,2
,
Mengyu Chen*
1,2
,
Guang Chen
3
,
Si Luo
,
Wen Xue
2
,
Xuelian Liu
2
,
Jiwu Wang
2,3
,
Ping Wei
1
Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School
,
University of South China
,
2
Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School
,
University of South China
,
3
Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School
,
University of South China
,
4
Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes
,
Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
Tags
CRISPR-based Modular Assembly
CRISPRmass
Two-step Test Tube Reactions
Cas9 SgRNA
CDNA/ORF Plasmids
Vector-specific UAS Module
Cas9 Linearized CDNA/ORF Plasmids
Single-step Reaction Assembly
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