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测量细菌细胞的抗弯刚度,使用光学陷阱
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap
Please note that all translations are automatically generated.
Click here for the English version.
测量细菌细胞的抗弯刚度,使用光学陷阱
DOI:
10.3791/2012-v
•
05:45 min
•
April 26, 2010
•
Siyuan Wang
,
Hugo Arellano-Santoyo
,
Peter A. Combs
,
Joshua W. Shaevitz
1
Department of Molecular Biology, Lewis-Sigler Institute for Integrative Genomics
,
Princeton University
,
2
Department of Physics, Lewis-Sigler Institute for Integrative Genomics
,
Princeton University
Chapters
00:00
Title
00:41
Introduction
01:12
Attaching Bacteria to a Coverlip and Preparing the Beads
03:19
Measuring the Bending Stiffness of the Bacterial Cells
05:11
Measuring the Bending Stiffness of Bacterial Cells Representative Results
05:21
Conclusion
05:44
Credits
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一个封面防滑表面附有一个光学陷阱来衡量细胞的抗弯刚度弯曲的丝状细菌细胞的协议。
Tags
Measuring
Bending Stiffness
Bacterial Cells
Optical Trap
Protocol
Filamentous Rod-shaped Bacteria
Forces
Microscopic Three-dimensional Spring
High-intensity Laser Beam
Chemically-treated Coverslip
Live Bacteria
Growing Ends
Filamentous Growth
Drug Cephalexin
Cell Attachment
Bending Forces
Polylysine-coated Bead
Force Measurement
Displacement Measurement
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