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Journal
/
Immunology and Infection
/
Staining and Imaging to Visualize Neutrophil Extracellular Traps
/
Autofluorescence Blocking and Antigen Retrieval in Tissue Samples
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Autofluorescence Blocking and Antigen Retrieval in Tissue Samples
Autofluorescence Blocking and Antigen Retrieval in Tissue Samples
Staining and Imaging to Visualize Neutrophil Extracellular Traps
DOI:
10.3791/200336-v
•
01:56 min
•
August 18, 2023
•
Lavinia Schoenfeld
,
Birgit Appl
,
Laia Pagerols-Raluy
,
Annika Heuer
,
Konrad Reinshagen
,
Michael Boettcher
2
1
Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf
,
University of Hamburg
,
2
Department of Pediatric Surgery, University Medical Center Mannheim
,
University of Heidelberg
,
3
Division of Spine Surgery, Department of Trauma and Orthopedic Surgery
,
University Medical Center Hamburg-Eppendorf (UKE)
Tags
Autofluorescence Blocking
Antigen Retrieval
Citrate Buffer
Target Retrieval Solution
TRS
PH
Deionized Water
Heat Treatment
Wet Chamber
Autofluorescence-reducing Agent
Ethanol
Antigen Retrieval
TRIS Buffered Saline
Tween
Hydrophobic Barrier Pen
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