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闪存冻结和Cryosectioning E12.5小鼠脑
JoVE Journal
Biology
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JoVE Journal
Biology
Flash Freezing and Cryosectioning E12.5 Mouse Brain
Please note that all translations are automatically generated.
Click here for the English version.
闪存冻结和Cryosectioning E12.5小鼠脑
DOI:
10.3791/198-v
•
28:32 min
•
May 28, 2007
•
D. Spencer Currle
,
Edwin S. Monuki
1
Department of Developmental and Cell Biology
,
University of California, Irvine (UCI)
Chapters
00:06
Introduction
00:18
Tools and reagents
01:23
Preparation of the cryomold
04:45
Orienting tissue
07:52
Flash freezing
09:39
Using cryostat
11:33
Preparation and mounting tissue for sectioning
19:33
Sectioning
27:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这个视频展示了闪光冻结和从小鼠胚胎脑组织切片技术。使用低温恒温器的有用提示,其中包括排除故障的方法,可以用来切割,以确保由此产生的组织部分无裂缝和其他扭曲。
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Flash Freezing
Cryosectioning
E12.5
Mouse Brain
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