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Biology
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大蛋白复合物结构质谱分析
JoVE Journal
Biology
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JoVE Journal
Biology
Analyzing Large Protein Complexes by Structural Mass Spectrometry
Please note that all translations are automatically generated.
Click here for the English version.
大蛋白复合物结构质谱分析
DOI:
10.3791/1954-v
•
15:35 min
•
June 19, 2010
•
Noam Kirshenbaum
,
Izhak Michaelevski
,
Michal Sharon
1
Department of Biological Chemistry
,
Weizmann Institute of Science
Chapters
00:00
Title
00:46
Introduction
01:18
Preparation of Gold-coated Capillaries for Nanoflow Electrospray Ionization
04:12
Calibrating the Mass Spectrometer for High Mass Measurements
07:47
MS Analysis of Intact Protein Complexes
10:45
Tandem Mass Spectrometry: Dissociating Protein Complexes
13:08
Representative Results of Large Protein Complexes Analysis by Mass Spectrometry
15:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
质谱法已被证明是一个有价值的工具,用于分析大型蛋白质复合物的。这种方法使多亚基组件组成,化学计量学和整体结构的见解。在这里,我们所描述的,一步一步,如何执行一个结构性的质谱分析,和特性的大分子结构。
Tags
Large Protein Complexes
Structural Mass Spectrometry
Biological Processes
Protein Organization
Macromolecular Complexes
Mass Spectrometry (MS)
Protein Composition
Subunit Stoichiometry
Structural Topology
High Sensitivity
Low Sample Requirement
Real-time Analysis
Separate Populations
Mixture Analysis
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