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Journal
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Biology
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真核Polyribosome剖面分析
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Eukaryotic Polyribosome Profile Analysis
Please note that all translations are automatically generated.
Click here for the English version.
真核Polyribosome剖面分析
DOI:
10.3791/1948-v
•
09:16 min
•
June 15, 2010
•
Anthony M. Esposito
,
Maria Mateyak
,
Dongming He
,
Marcus Lewis
,
Arjun N. Sasikumar
,
Jenna Hutton
,
Paul R. Copeland
,
Terri G. Kinzy
1
Department of Molecular Genetics, Microbiology, and Immunology
,
University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School
Chapters
00:45
Introduction
01:15
Preparation of Sucrose Gradients
02:53
Preparation of Extracts from Yeast Cells
05:06
Centrifugation of Gradients and Collection of Data and Fractions
08:35
Representative Trace of a Ribosome Extract
08:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了协议的真核细胞的核糖体翻译的提取。一旦提取,核糖体是由蔗糖梯度分馏分离到monosomes和多聚核糖体,允许不同的核糖体的人群进行分析。因此,这种方法是研究翻译调控的黄金标准。
Tags
Eukaryotic
Polyribosome Profile Analysis
Protein Synthesis
Translation Regulation
MRNA/ribosome Complexes
Sucrose Density Gradient
Polyribosomes
Monosomes
Translation Initiation
Elongation/termination
MRNA Cohort
Experimental Conditions
Ribosome Assembly
Small Ribosomal Subunit
Large Ribosomal Subunit
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