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Journal
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Biology
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人脐动脉平滑肌细胞的分离(HUASMC)
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JoVE Journal
Biology
Isolation of Human Umbilical Arterial Smooth Muscle Cells (HUASMC)
Please note that all translations are automatically generated.
Click here for the English version.
人脐动脉平滑肌细胞的分离(HUASMC)
DOI:
10.3791/1940-v
•
08:06 min
•
July 03, 2010
•
Maximiano P. Ribeiro
,
Ricardo Relvas
,
Samuel Chiquita
,
Ilídio J. Correia
1
Centro de Investigação em Ciências da Saúde, Faculdade de Ciências da Saúde
,
Universidade da Beira Interior
Chapters
00:00
Title
00:06
Solutions
00:48
Preparation of Umbilical Cords
02:40
Digestion of Umbical Arteries and Isolation of Smooth Muscle Cells
04:51
Culture
08:00
Credits
Summary
Automatic Translation
English (Original)
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Automatic Translation
脐带是用来隔离平滑肌细胞,通过不同的方式。在这项工作中,我们使用的孤立的平滑肌细胞的酶处理。
Tags
Isolation
Human Umbilical Arterial Smooth Muscle Cells
HUASMC
Umbilical Cord
Biological Sample
Endothelial Cells
Smooth Muscle Cells
Blood Vessels
Enzymatic Treatment
Experimental Procedure
Cell Isolation
T-flash
DMEM-F12
Fetal Bovine Serum
Culture
Passages
Morphological Characteristics
Phenotypic Characteristics
Constrictor Drugs
L-type Calcium Channels
Vascular Function
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