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Biology
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MALDIサンプル調製:超薄層法
JoVE Journal
Biology
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JoVE Journal
Biology
MALDI Sample Preparation: the Ultra Thin Layer Method
Please note that all translations are automatically generated.
Click here for the English version.
MALDIサンプル調製:超薄層法
DOI:
10.3791/192-v
•
05:28 min
•
April 29, 2007
•
David Fenyo
,
Qingjun Wang
,
Jeffrey A. DeGrasse
,
Julio C. Padovan
,
Martine Cadene
,
Brian T. Chait
1
Laboratory of Mass Spectrometry and Gaseous Ion Chemistry
,
Rockefeller University
Chapters
00:20
Step 1: cleaning of the sample plate
00:46
Step 2: preparation of the thin layer substrate solution
00:58
Step 3: making the thin layer substrate
01:52
Step 4: preparation of the matrix solution
02:05
Step 5: test of the thin layer
02:50
Step 6: sample application
03:47
Step 7: calibrant application
04:20
Step 8: washing step
05:05
Step 9: acquisition of mass spectra
Summary
Automatic Translation
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Automatic Translation
このビデオでは、マトリックス支援レーザー脱離イオン化質量分析法(MALDI - MS)でペプチドやタンパク質を解析するための超薄型行列/分析物層の準備を示しています。
Tags
MALDI
Sample Preparation
Ultra Thin Layer Method
Matrix-Assisted Laser Desorption Ionization Mass Spectrometry
Matrix Crystals
Alpha-cyano-4-hydroxycinnamic Acid
Substrate Layer
Crystallization
Advantages
Greater Tolerance To Impurities
Better Resolution
Higher Spatial Uniformity
Accurate Mass Determination
Membrane Proteins
Ion Channels
Metabolite Transporters
Receptors
Transmembrane Domains
Soluble Proteins
Molecular Masses
High-quality Spectra
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