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使用自动细胞计数器简化基因表达研究:在Namalwa细胞的IL - 4依赖的基因表达的siRNA击倒
JoVE Journal
Biology
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JoVE Journal
Biology
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Please note that all translations are automatically generated.
Click here for the English version.
使用自动细胞计数器简化基因表达研究:在Namalwa细胞的IL - 4依赖的基因表达的siRNA击倒
DOI:
10.3791/1904-v
•
10:34 min
•
April 14, 2010
•
Adam M. McCoy
,
Claudia Litterst
,
Michelle L. Collins
,
Luis A. Ugozzoli
1
Gene Expression Division
,
Bio-Rad Laboratories
Chapters
00:00
Title
00:49
Intro
01:16
Preparing and Counting the Namalwa Cells
04:17
Electroporating the Cells in 96-well Plates
05:55
Extract and Check RNA
07:54
Real-time PCR
08:58
Analysis of Gene Expression
10:03
Conclusion
Summary
Automatic Translation
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Automatic Translation
此过程介绍了一个快速简便的工作流程,引入困难的siRNA转染细胞系,并按照实时PCR基因表达。使用自动细胞计数器,多井的电板,全自动电泳站提供快速和可靠的结果,而不需要昂贵的机械手。
Tags
Automated Cell Counter
Gene Expression Studies
SiRNA Knockdown
IL-4 Dependent Gene Expression
Namalwa Cells
Cellular Models
Electroporation
Nucleic Acids Transfection
Signaling Pathway
Experimental Stages
Post Transfection Real-time PCR Gene Expression Analysis
Transcriptional Activator STAT6
CCL17
Burkitt Lymphoma Cell Line
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