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在芽殖酵母中的蛋白浓度的功能形态学表型的发展分析
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Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast
Please note that all translations are automatically generated.
Click here for the English version.
在芽殖酵母中的蛋白浓度的功能形态学表型的发展分析
DOI:
10.3791/1863-v
•
15:02 min
•
March 24, 2010
•
Debarati Mukherjee
,
Arpita Sen
,
R. Claudio Aguilar
1
Department of Biological Sciences and Purdue Center for Cancer Research
,
Purdue University
Chapters
00:00
Title
00:49
Introduction
01:30
Cell Culture and Protein Induction
04:00
Quantification of Cell-Division Phenotype and Cell Death
05:32
Visualizing Phenotype-Specific Defects by Fluorescence Microscopy
07:58
Determination of Phenotype Development at the Single Cell Level by Time-Lapse Video Microscopy
11:55
Effects of ENTH2 Overexpression
14:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
基因缺失和蛋白表达研究蛋白质功能的常用方法。在这篇文章中,我们描述了作为一个人口功能的蛋白浓度和单细胞水平分析表型的发展协议<em>酿酒酵母</em>。
Tags
Morphological Phenotype
Protein Concentration
Budding Yeast
Gene Deletion
Protein Overexpression
Phenotype Development
Population Level
Single-cell Level
Saccharomyces Cerevisiae
Endocytic Adaptor Protein
Epsin
Dominant Negative Approach
ENTH Domain
Cell Division Defect
Mislocalization Of Scaffolding Proteins
Septins
Article
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