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分离和富集大鼠间质干细胞(MSCS)及衍生的干细胞分离单细胞集落
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Biology
Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs
Please note that all translations are automatically generated.
Click here for the English version.
分离和富集大鼠间质干细胞(MSCS)及衍生的干细胞分离单细胞集落
DOI:
10.3791/1852-v
•
08:18 min
•
March 22, 2010
•
Linxia Zhang
,
Christina Chan
1
Department of Chemical Engineering and Materials Science
,
City of Hope Cancer Center
Chapters
00:00
Title
00:47
Introduction
01:18
Isolation of Rat MSCs
02:40
Enrichment of Rat MSCs
05:17
Separation of Single-colony Derived MSCs
06:36
Results of Isolation and Enrichment of Rat MSCs
07:41
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
大鼠MSCs从股骨和胫骨分离,然后通过磁性细胞分选富集。排序细胞被证实为流式细胞仪检测表面标志物表达。这些细胞克隆密度培养,形成单菌落,然后这些菌落克隆气瓶分开。
Tags
Isolation
Enrichment
Rat Mesenchymal Stem Cells
MSCs
Single-colony Derived MSCs
Bone Marrow
Hematopoietic Stem Cells
Plastic Adherence
Protocol
Rat Femurs
Tibias
Magnetic Cell Sorting
Surface Markers
CD54
CD90
Flow Cytometry Analysis
HSC Marker CD45
Heterogeneity
Subpopulations
Cloning Cylinders
Culture Evaluation
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