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収穫精子とマウスの人工授精
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Biology
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JoVE Journal
Biology
Harvesting Sperm and Artificial Insemination of Mice
Please note that all translations are automatically generated.
Click here for the English version.
収穫精子とマウスの人工授精
DOI:
10.3791/184-v
•
10:00 min
•
April 28, 2007
•
Amanda R. Duselis
,
Paul B. Vrana
1
Dept. of Biological Chemistry
,
University of California, Irvine (UCI)
Chapters
00:06
Introduction
01:09
Tools and Reagents
02:17
Dissection procedure
04:43
Removal of epididymis
07:58
Visualization of sperm
08:20
Insemination of females
09:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルは、男性の精巣から精子を抽出し、雌マウスをinseminatingための方法を示しています。正確な時間が発生試験と同様にトランスジェニック作業に必要な場合にこの手順が便利です。
Tags
Sperm Harvesting
Artificial Insemination
Mice
Peromyscus Species
Research
Model
Protocol
Testes Extraction
Sperm Isolation
Milk Mixture
Female Reproductive Tract
Fertilization
Embryo Development
Embryo Timing
Rodent Species
Mating Assessment
Transgenic Work
Reduced Animal Usage
Peromyscus Sy
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