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微流体调查可溶性信号介导的相互作用的上皮细胞和细菌的共同文化
JoVE Journal
Biology
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JoVE Journal Biology
Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions
DOI:

12:25 min

April 20, 2010

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Chapters

  • 00:00Title
  • 00:42Introduction
  • 01:29Preparation of Replica Molds from Master Silicone Wafer
  • 04:00Bonding, Assemebling and Preparing Multilayer PDMS Devices
  • 06:55Cultivation of Commensal E.coli Biofilm
  • 08:05Introduction of Eukaryotic Cells Around Commensal Bacteria
  • 10:55Modeling the Gut Bacterial Infection Environment Using a Tri-Layer Microfluidic Device
  • 11:40Conclusion

Summary

Automatic Translation

这个协议描述为上皮细胞和细菌的同时,本地化的文化的微流体的共培养模型。该模型可用于调查发病机制不同的可溶性的分子信号的作用,以及屏幕上公认的益生菌株细菌的效力。

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