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微流体调查可溶性信号介导的相互作用的上皮细胞和细菌的共同文化
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Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions
Please note that all translations are automatically generated.
Click here for the English version.
微流体调查可溶性信号介导的相互作用的上皮细胞和细菌的共同文化
DOI:
10.3791/1749-v
•
12:25 min
•
April 20, 2010
•
Jeongyun Kim
,
Manjunath Hegde
,
Arul Jayaraman
2
1
McFerrin Department of Chemical Engineering
,
Texas A&M University
,
2
Department of Biomedical Engineering
,
Texas A&M University
Chapters
00:00
Title
00:42
Introduction
01:29
Preparation of Replica Molds from Master Silicone Wafer
04:00
Bonding, Assemebling and Preparing Multilayer PDMS Devices
06:55
Cultivation of Commensal E.coli Biofilm
08:05
Introduction of Eukaryotic Cells Around Commensal Bacteria
10:55
Modeling the Gut Bacterial Infection Environment Using a Tri-Layer Microfluidic Device
11:40
Conclusion
Summary
Automatic Translation
English (Original)
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italiano (Italian)
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Automatic Translation
这个协议描述为上皮细胞和细菌的同时,本地化的文化的微流体的共培养模型。该模型可用于调查发病机制不同的可溶性的分子信号的作用,以及屏幕上公认的益生菌株细菌的效力。
Tags
Microfluidic Co-culture
Epithelial Cells
Bacteria
Soluble Signals
Interactions
Gastrointestinal Tract
Commensal Bacteria
Pathogen Colonization
Culture Methods
Microenvironment
Microfluidic Co-culture Model
Eukaryotic Cells
Escherichia Coli Biofilm
Enterohemorrhagic E. Coli (EHEC)
GI Tract Infection
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