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Journal
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Immunology and Infection
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多色流流式细胞仪分析细胞在恒河猴的免疫应答
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques
Please note that all translations are automatically generated.
Click here for the English version.
多色流流式细胞仪分析细胞在恒河猴的免疫应答
DOI:
10.3791/1743-v
•
07:11 min
•
April 22, 2010
•
Hong He
,
Amy N. Courtney
,
Eric Wieder
,
K. Jagannadha Sastry
1
Department of Immunology
,
MD Anderson Cancer Center – University of Texas
,
2
Department of Medicine
,
University of Miami
Chapters
00:00
Title
00:46
Introduction
01:12
Antigenic Stimulation
02:38
Immunofluorescent Staining of Surface Markers for FACS
04:25
Intracellular Cytokine Staining
05:27
Representative FACS Data
06:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们展示了总额以及记忆CD4 +和CD8 + T细胞在猕猴,艾滋病毒/艾滋病疫苗研究的理想模型的子集的详细的表型和功能特性的多色流式细胞仪的实用。
Tags
Multicolor Flow Cytometry
Cellular Immune Response
Rhesus Macaques
HIV-AIDS Model
Pathogenesis
Vaccines
Therapeutics
Phenotypic Analysis
Functional Analysis
Intracellular Cytokine Production
CD4+ T Cells
CD8+ T Cells
Memory Subsets
Interferon Gamma (INF-)
Interleukin (IL)-2
PMA Plus Ionomycin (PMA+I)
Cytokine Profiles
Memory Cells Subsets
Antigen-specific CD4+ T Cells
Antigen-specific CD8+ T Cells
PBMC Samples
HIV Envelope Peptide Cocktail Vaccine
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