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Journal
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Immunology and Infection
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激活的B细胞CD40的小鼠模型
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Murine Model of CD40-activation of B cells
Please note that all translations are automatically generated.
Click here for the English version.
激活的B细胞CD40的小鼠模型
DOI:
10.3791/1734-v
•
12:24 min
•
March 05, 2010
•
Tanja M. Liebig
,
Anne Fiedler
,
Nela Klein-Gonzalez
,
Alexander Shimabukuro-Vornhagen
,
Michael von Bergwelt-Baildon
1
Laboratory for Tumor and Transplantation Immunology, Department I of Internal Medicine
,
University Hospital of Cologne
Chapters
00:00
Title
00:14
Introduction
02:10
Preparation of Murine Splenocytes for CD40 – Stimulation
04:19
Preparation of Feeder Cells
07:59
CD40 – Stimulation
08:50
Subcultivation
11:25
Analysis of Murine CD40 – Activated B Cells
11:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这个视频中,我们展示的CD40从C57BL / 6小鼠的脾细胞,它可以用来作为模型抗原提呈细胞(APC)的研究免疫诱导小鼠的B细胞的活化和扩大的过程。
Tags
CD40-activation
B Cells
Antigen Presentation
Interleukin-4
CD40 Ligand
Expansion
Peripheral Blood
CD40-B Cells
Lymph Node Homing Molecules
T Cells
Antigens
Memory T Cells
Immuno-stimulatory Antigen-presenting Cells (APC)
Cell-based Immunotherapy
Murine CD40-activated B Cells
Splenocytes
Purified B Cells
C57BL/6 Mice
Haplotype H-2b
Density Gradient Centrifugation
HeLa Cells
Recombinant Murine CD40 Ligand (tmuCD40L HeLa)
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