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KLRG1テトラマーの生産のためのプロトコル
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Biology
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JoVE Journal
Biology
A Protocol for the Production of KLRG1 Tetramer
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KLRG1テトラマーの生産のためのプロトコル
DOI:
10.3791/1701-v
•
07:24 min
•
January 12, 2010
•
Stephanie C. Terrizzi
,
Cindy Banh
,
Laurent Brossay
1
Department of Molecular Microbiology and Immunology
,
Brown University
Chapters
00:00
Title
00:08
Introduction
00:33
Preparation of Inclusion Bodies
03:07
Refolding of KLRG1
04:41
Concentration and Purification of Refolded KLRG1 Tetramers
06:25
Size Exclusion Chromatography of Refolded KLRG1
06:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルは、KLRG1リガンドの分析のための強力なツールですKLRG1四量体、の製造を説明します。
Tags
KLRG1
Tetramer Production Protocol
Killer Cell Lectin-like Receptor G1
C Type Lectin-like Superfamily
Monomer
Disulfide-linked Homodimer
NK Cells
Effector/memory T Cells
KLRG1 Ligands
E-cadherin
N-cadherin
R-cadherin
Ca2+-dependent Cell-cell Adhesion Molecules
Cadherin Repeats
Transmembrane Domain
Cytoplasmic Domain
Actin Cytoskeleton
Immune Response
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