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现场剖析果蝇胚胎抗体染色筛选突变体集合的简化方法
JoVE Journal
Biology
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JoVE Journal Biology
Live Dissection of Drosophila Embryos: Streamlined Methods for Screening Mutant Collections by Antibody Staining
DOI:

13:48 min

December 29, 2009

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Chapters

  • 00:00Title
  • 01:21Introduction
  • 02:14Collection of Embryos From Fly Crosses
  • 04:15Staging Embryos and Sorting for GFP Expression
  • 06:34Drosophila Embryo Live Dissection
  • 08:31Embryo Fixation and Antibody Staining
  • 12:11Immunofluoresent Imaging of Dissected Drosophila Embryos
  • 12:58Conclusion

Summary

Automatic Translation

我们描述了一个用于生成特定基因型的果蝇胚胎的“鱼片”筹备工作的精简的协议。此协议允许多种遗传筛选的高效执行。它也可以让优秀的中后期的胚胎结构的可视化。

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