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Journal
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Biology
/
现场剖析
果蝇
胚胎抗体染色筛选突变体集合的简化方法
JoVE Journal
Biology
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JoVE Journal
Biology
Live Dissection of
Drosophila
Embryos: Streamlined Methods for Screening Mutant Collections by Antibody Staining
Please note that all translations are automatically generated.
Click here for the English version.
现场剖析
果蝇
胚胎抗体染色筛选突变体集合的简化方法
DOI:
10.3791/1647-v
•
13:48 min
•
December 29, 2009
•
Hyung-Kook (Peter) Lee
,
Ashley P. Wright
,
Kai Zinn
1
Division of Biology
,
California Institute of Technology
Chapters
00:00
Title
01:21
Introduction
02:14
Collection of Embryos From Fly Crosses
04:15
Staging Embryos and Sorting for GFP Expression
06:34
Drosophila Embryo Live Dissection
08:31
Embryo Fixation and Antibody Staining
12:11
Immunofluoresent Imaging of Dissected Drosophila Embryos
12:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个用于生成特定基因型的果蝇胚胎的“鱼片”筹备工作的精简的协议。此协议允许多种遗传筛选的高效执行。它也可以让优秀的中后期的胚胎结构的可视化。
Tags
Live Dissection
Drosophila Embryos
Screening Mutant Collections
Antibody Staining
Fillet Preparations
Central Nervous System
Body Walls
Fixed Dissections
Mutant Embryos
GFP Balancer Chromosomes
Deficiency Screens
Ectopic Expression Screens
Orphan Receptors
Streamlined Protocols
Live Embryo Dissection
Large Collections Of Stocks
Phenotypes
Compound Microscope
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