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根据流量的血小板粘附和聚集,使用微流控流细胞
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
Please note that all translations are automatically generated.
Click here for the English version.
根据流量的血小板粘附和聚集,使用微流控流细胞
DOI:
10.3791/1644-v
•
10:10 min
•
October 27, 2009
•
Carolyn G. Conant
,
Michael A. Schwartz
,
Tanner Nevill
,
Cristian Ionescu-Zanetti
1
Fluxion Biosciences, Inc.
Chapters
00:00
Title
00:57
Introduction
01:16
Preparation of the Microfluidic Channels of a BioFlux Plate
04:34
Preparing the Blood with GPIIb/IIIa Inhibitory Antibody
05:41
Running the Flow Cell Experiment on the BF1000 Workstation
08:19
Platelet Adhesion and Aggregation Results
08:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
血小板粘附级联剪切流的存在,这个因素不占在常规(静态)以及板的检测。本文报道利用一个微井板格式,模拟生理剪切流条件的检测血小板聚集。
Tags
Platelet Adhesion
Platelet Aggregation
Microfluidic Flow Cells
Shear Flow
Well-plate Assays
Microfluidic Well-plate Format
Physiological Shear Flow
Extracellular Proteins
Collagen I
Von Willebrand Factor
Active Perfusion
Pneumatic Pump
Buffer
Platelet Interactions
Fluorescent Dye
Flow Rates
Platelet Activation
Platelet Aggregation Inhibitors
IC50 Dose Response Data
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