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在体内的器官型小鼠视网膜Wholemount文化
JoVE Journal
Biology
Author Produced
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JoVE Journal Biology
In vivo-like Organotypic Murine Retinal Wholemount Culture
DOI:

25:26 min

January 11, 2010

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Chapters

  • 00:00Title
  • 00:35Introduction
  • 01:50Enucleation of Murine Eyes and Dissection of Retinas
  • 04:02Dissection of Embryonic, Postnatal and Adult Murine Retinas
  • 11:40Murine Organotypic Retinal Wholemount Culture
  • 14:10Dissociation of Cultured Retinal Wholemounts
  • 19:35Washing of Dissociated Cell Suspensions
  • 24:49Conclusion

Summary

Automatic Translation

此视频文章演示器官视网膜wholemount文化和cytospin一个外生的诱导效应分析程序的建立。器官型视网膜wholemount文化模仿<em>在体内</em>情况,同时规避经典的小鼠动物模型的缺点,大大促进了实验操作的小鼠视网膜的无障碍。

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