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Journal
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Biology
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分离纯化
果蝇
外周神经元
JoVE Journal
Biology
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JoVE Journal
Biology
Isolation and Purification of
Drosophila
Peripheral Neurons by Magnetic Bead Sorting
Please note that all translations are automatically generated.
Click here for the English version.
分离纯化
果蝇
外周神经元
DOI:
10.3791/1599-v
•
12:04 min
•
December 01, 2009
•
Eswar Prasad R. Iyer
2
,
Srividya Chandramouli Iyer
2
,
Mikolaj J. Sulkowski
2
,
Daniel N. Cox
2
1
Department of Molecular and Microbiology
,
George Mason University
,
2
Krasnow Institute for Advanced Study
,
George Mason University
Chapters
00:00
Title
00:58
Introduction
01:31
Selecting and Washing Larvae
02:27
Dissecting the Larvae and Removing Non-Specific Cells
04:47
Dissociating the Tissue into a Single Cell Suspension
08:07
Magnetic Bead Cell Sorting
10:05
Representative Results of DA Neurons Isolation and RNA Extraction
11:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这个视频文章中,我们目前的分离纯化的方法<em>果蝇</em>使用快速磁珠辅助细胞排序策略外周神经元。从分离的细胞获得的RNA可以很容易地用于下游应用,包括微阵列分析。
Tags
Isolation
Purification
Drosophila Peripheral Neurons
Magnetic Bead Sorting
PNS Neurons
Dendritic Arborization (da) Neurons
Neuronal Development
Dendrite Morphogenesis
Functional And Molecular Levels
Pure Population
Low Occurrence
Tough Chitinous Larval Cuticle
Specific Cell Enrichment
Antibody-coated Magnetic Beads
Age-matched Third Instar Larvae
Mouse CD8 Tagged GFP Fusion Protein (UAS-mCD8-GFP)
Pickpocket (ppk)-GAL4 Driver
Pan-da Neuron-specific GAL421-7 Driver
Larval Cuticle
Cell Types
Article
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