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超高分子量基因组DNA的提取土壤和沉积物
JoVE Journal
Biology
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JoVE Journal
Biology
Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
Please note that all translations are automatically generated.
Click here for the English version.
超高分子量基因组DNA的提取土壤和沉积物
DOI:
10.3791/1569-v
•
11:24 min
•
November 10, 2009
•
Sangwon Lee
,
Steven J. Hallam
1
Department of Microbiology and Immunology
,
University of British Columbia – UBC
Chapters
00:00
Title
00:08
Introduction
00:53
Cell Lysis
02:21
DNA Extraction
08:51
Cesium Chloride Gradient Ultracentrifugation
09:46
Representative Results/ Outcome
10:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一个隔离土壤微生物群落的超高分子量和高品质的基因组DNA的方法来描述。
Tags
High Molecular Weight Genomic DNA
Soils
Sediments
Soil Microbiome
Metagenomic Approaches
Genetic Information
Pathway Reconstruction
Functional Screening
Therapeutic Processes
Biomass Conversion Processes
Large Insert Library Production
Protocol
Cell Lysis
Mechanical Forces
Chemical Forces
Genomic DNA Extraction
Extraction Buffer
Chloroform-isoamyl Alcohol
Isopropyl Alcohol
Guanidine Isothiocyanate
Hexadecyltrimethylammonium Bromide (CTAB)
Integrity Preservation
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