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Biology
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慢病毒生产
JoVE Journal
Biology
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JoVE Journal
Biology
Lentivirus Production
Please note that all translations are automatically generated.
Click here for the English version.
慢病毒生产
DOI:
10.3791/1499-v
•
11:42 min
•
October 02, 2009
•
Xiaoyin Wang
,
Michael McManus
1
Diabetes Center
,
University of California, San Francisco – UCSF
Chapters
00:00
Title
00:46
Introduction
01:15
Transfection of 293 Cells To Produce Lentivirus
03:24
Lentivirus Harvest
04:39
Lentivirus Concentration via Ultracentrifugation
07:24
Lentivirus Titration Using Flow Cytometry
11:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
为了使慢病毒,DNA载体转染到人293细胞。收获后,集中上清液,荧光表达,流式细胞仪,是由病毒滴度。
Tags
Lentivirus Production
RNA Interference
Gene Silencing
Short Interfering RNAs (siRNA)
Post-transcriptional State
Short Hairpin RNAs
Lentiviruses
Packaging Lentiviruses
Lentiviral Vector Production Systems
Helper Plasmid Constructs
Replication-capable Virus
Transient Transfection
Human 293 Cells
Incubation Period
Virus Titer
Reporter Expression
Flow Cytometer
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