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Chapters
- 00:05Title
- 00:41Introduction
- 01:03Extraction of Total RNA using TRIzol Reagent
- 03:55RNA Cleanup using the Qiagen RNEasy Mini Kit
- 07:57cDNA Synthesis using the Invitrogen SuperScript First-Strand System
- 10:28cDNA Isolation and Precipitation
- 13:50Representative Results/Outcome
- 14:57Conclusion
De isolatie van hoge kwaliteit, intact RNA is een essentiële stap in veel laboratoria protocollen. Hier laten we zien RNA-extractie uit hele zebravis embryo's en cDNA synthese voor verdere toepassing in de verschillende experimentele procedures, zoals het gen expressie microarray analyse.
Tags
RNA IsolationEmbryonic ZebrafishCDNA SynthesisGene Expression AnalysisHigh Quality RNAIntact RNADegraded SampleImpuritiesSolid TechniquesSafeguardsQuality Control ChecksSuperior SampleCommercial RNA Isolation KitChemical DenaturantDNA RemovalRNA StabilityCDNA Synthesis KitCleavage By RNAsesGene Expression AssayStable CDNA ProductMicroarray AnalysisRT-PCRLong-term Storage
