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成人的心肌细胞的分离和遗传操作共聚焦成像
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging
DOI:

12:15 min

September 17, 2009

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Chapters

  • 00:00Title
  • 00:37Introduction
  • 01:18Isolation of Adult Cardiac Myocytes from Rat Heart by Langendorrf Preparation
  • 08:02Primary Cell Culture and Adenoviral Transduction
  • 09:45Confocal Imaging of 3D Subcellular Structures
  • 11:09Imaging of Adult Rat Cardiac Myocytes Representative Results
  • 11:43Conclusion

Summary

Automatic Translation

成人的心肌细胞原代细胞,可以从动物心脏中分离和培养几天。在这个文化时期的腺病毒介导的基因转移,可以用来表达基因编码的生物传感器(GEBs)或荧光融合蛋白。这两种方法都可以通过共聚焦显微镜细胞调查。

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