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成人的心肌细胞的分离和遗传操作共聚焦成像
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Biology
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JoVE Journal
Biology
Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging
Please note that all translations are automatically generated.
Click here for the English version.
成人的心肌细胞的分离和遗传操作共聚焦成像
DOI:
10.3791/1433-v
•
12:15 min
•
September 17, 2009
•
Lars Kaestner
,
Anke Scholz
,
Karin Hammer
,
Anne Vecerdea
,
Sandra Ruppenthal
,
Peter Lipp
1
Institute for Molecular Cell Biology
,
Universty of Saarland
Chapters
00:00
Title
00:37
Introduction
01:18
Isolation of Adult Cardiac Myocytes from Rat Heart by Langendorrf Preparation
08:02
Primary Cell Culture and Adenoviral Transduction
09:45
Confocal Imaging of 3D Subcellular Structures
11:09
Imaging of Adult Rat Cardiac Myocytes Representative Results
11:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
成人的心肌细胞原代细胞,可以从动物心脏中分离和培养几天。在这个文化时期的腺病毒介导的基因转移,可以用来表达基因编码的生物传感器(GEBs)或荧光融合蛋白。这两种方法都可以通过共聚焦显微镜细胞调查。
Tags
Isolation
Genetic Manipulation
Adult Cardiac Myocytes
Confocal Imaging
Embryonic Cells
Neonatal Muscle Cells
Working Heart
Cardiac Cellular Physiology
Pathophysiology
Pharmaceutical Investigations
Transgenic Animal Models
Long Term Culture
Adenoviral Gene Transfer
Genetically Encoded Biosensors
Fluorescent Fusion Proteins
Protein Overexpression
Protein Downregulation
RNAi
Subcellular Structure Staining
Golgi Visualization
Confocal Imaging
Z-stacks Reconstruction
Cellular Cardiology
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