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快速,灵敏的胶体考马斯亮G - 250染色蛋白聚丙烯酰胺凝胶
JoVE Journal
Biology
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JoVE Journal Biology
Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels
DOI:

10:57 min

August 03, 2009

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Chapters

  • 00:00Title
  • 00:55Introduction
  • 01:58Two-Dimensional (2-D) Gel Electrophoresis Using Cup-Loading
  • 06:30Colloidal Coomassie staining with CBB G-250
  • 09:21Representative Results
  • 09:47Conclusion

Summary

Automatic Translation

该视频应普及胶体考马斯亮蓝G - 250染色协议,根据康<em>等。</em平均4凝胶吴蛋白质检测。染色完成后2小时内,没有任何努力。我们经常使用凝胶为基础的蛋白质组学分析的目的在康有为的协议。

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