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快速,灵敏的胶体考马斯亮G - 250染色蛋白聚丙烯酰胺凝胶
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JoVE Journal
Biology
Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels
Please note that all translations are automatically generated.
Click here for the English version.
快速,灵敏的胶体考马斯亮G - 250染色蛋白聚丙烯酰胺凝胶
DOI:
10.3791/1431-v
•
10:57 min
•
August 03, 2009
•
Nadine Dyballa
,
Sabine Metzger
1
Biological Medical Research Center (BMFZ)
,
University Duesseldorf
Chapters
00:00
Title
00:55
Introduction
01:58
Two-Dimensional (2-D) Gel Electrophoresis Using Cup-Loading
06:30
Colloidal Coomassie staining with CBB G-250
09:21
Representative Results
09:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该视频应普及胶体考马斯亮蓝G - 250染色协议,根据康<em>等。</em平均4凝胶吴蛋白质检测。染色完成后2小时内,没有任何努力。我们经常使用凝胶为基础的蛋白质组学分析的目的在康有为的协议。
Tags
Coomassie Brilliant Blue G-250
Protein Staining
SDS-PAGE
Silver Staining
Fluorescence Staining
Mass Spectrometric Protein Identification
Analytical Gel-based Proteomic Approaches
Sensitivity Enhancement
Low-abundant Proteins
Colloidal Particles
Methanol
Ammonium Sulfate
Phosphoric Acid
Blue Silver Staining
Kang Et Al.
Aluminum Sulfate
Ethanol
Article
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