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Journal
/
Biology
/
荧光标记的
果蝇
心结构
JoVE Journal
Biology
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JoVE Journal
Biology
Fluorescent Labeling of
Drosophila
Heart Structures
Please note that all translations are automatically generated.
Click here for the English version.
荧光标记的
果蝇
心结构
DOI:
10.3791/1423-v
•
11:12 min
•
October 13, 2009
•
Nakissa N. Alayari
2
,
Georg Vogler
,
Ouarda Taghli-Lamallem
,
Karen Ocorr
,
Rolf Bodmer
,
Anthony Cammarato
2
1
Biology Department
,
San Diego State University
,
2
Development and Aging Program, NASCR Center
,
The Sanford Burnham Institute for Medical Research
Chapters
00:00
Title
00:10
Introduction
01:50
Preparation and Dissection
06:33
Mounting Hearts from Adult Flies
08:15
Mounting Larval Hearts
09:35
Fluorescent Labeling of Drosophila Heart Structures: Results
10:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了从幼虫和成虫心管的不同元素的荧光标记的一个基本协议<em>果蝇</em>。这些标本非常适合通过荧光或共聚焦显微镜成像。这种技术允许一个强大的模式生物的心功能的详细的结构分析。
Tags
Fluorescent Labeling
Drosophila Heart Structures
Drosophila Melanogaster
Dorsal Vessel
Cardiomyocytes
Pericardial Cells
Alary Muscles
Ventral Longitudinal Muscle Cells
Myofibrils
Cytoskeletal/submembranous Protein Complexes
Extracellular Matrix
Fixation
Fluorescent Labeling Protocol
Sarcomeric F-actin
α-actinin
Myosin-GFP Expressing Adult Flies
Pericardin
Type IV Extracellular Matrix Collagen
Mounting Strategy
Semi-intact Adult Hearts
Imaging
Confocal Microscopy
Article
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