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Yüksek Saflaştırılmış Alınması Toxoplasma gondii Süreksiz Sezyum Klorür Gradient ookistleri
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient
DOI:

11:17 min

November 03, 2009

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Chapters

  • 00:00Title
  • 01:05Introduction
  • 01:35General Safety When Working With T. gondii Oocysts
  • 03:15Protocol Preparation and the Sucrose Float
  • 07:27CsCl Gradient
  • 10:03Resulting Oocysts
  • 10:34Conclusion

Summary

Automatic Translation

This study describes the development of a modified CsCl method that easily purifies T. gondii oocysts from feces of infected cats that are suitable for molecular biological and tissue culture manipulation

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