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无有机溶剂和醋酸，蛋白质与考马斯亮蓝G - 250凝胶染色
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JoVE Journal Biology
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
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无有机溶剂和醋酸，蛋白质与考马斯亮蓝G - 250凝胶染色

DOI:
10.3791/1350-v

•

07:47 min

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August 14, 2009

•

,
1Protein Expression and Purification Core Facility,EMBL Heidelberg

Chapters

  • 00:05Title
  • 01:02Introduction
  • 01:34Preparation of the CBB Staining Solution
  • 02:38SDS-PAGE
  • 04:26Staining of the Gel
  • 06:38Representative Results / Outcome
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

蛋白质与考马斯亮蓝（CBB）的G - 250在聚丙烯酰胺凝胶染色的一个短的协议是在古典与CBB染色程序，而无需使用有机溶剂或醋酸。

Tags

ProteinsGelsCoomassie G-250StainingOrganic SolventAcetic AcidSDS-PAGEMicrowave OvenToxic SolventsSafety ConsiderationsAlternative CompositionBidistilled WaterHClWashingHeating GelProtein Staining

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