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人类胰岛的分离:第二部分:人类胰岛的分离纯化及文化
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JoVE Journal
Biology
Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
Please note that all translations are automatically generated.
Click here for the English version.
人类胰岛的分离:第二部分:人类胰岛的分离纯化及文化
DOI:
10.3791/1343-v
•
08:35 min
•
May 26, 2009
•
Meirigeng Qi
,
Barbara Barbaro
,
Shusen Wang
,
Yong Wang
,
Mike Hansen
,
Jose Oberholzer
1
Department of Surgery
,
University of Illinois, Chicago
Chapters
00:00
Title
00:32
Introduction
01:05
Purification of Islet
05:03
Sampling and Culture
07:08
Representative Outcome: Evaluate Islet Purity and Continue
07:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
实现高品质和适当数量的人类胰岛是胰岛移植成功的突出的先决条件之一。在这个视频中,我们描述一步一步人类胰岛隔离(第二部分:人类胰岛的纯化和文化)的程序,采用改良的自动方法。
Tags
Human Pancreatic Islet Isolation
Purification
Culture
Type 1 Diabetes
Glucose Control
Insulin Therapy
Islet Transplantation
Donor Characteristics
Organ Procurement
Preservation
Isolation Outcome
UIC (University Of Illinois At Chicago)
Isolation Protocol
Purification Gradient
Cold Preservation Solutions (UW
HTK)
Cell Isolation Laboratory
UIC Method
Ricordi Et Al Method
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