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Chapters
- 00:04Title
- 00:52Introduction
- 01:18Preparation for Surgery
- 04:06The Rat Heart Dissection
- 05:05Myocytes Isolation
- 10:15Myocyte Cell Culture
- 10:56Representative Outcome: Cultured Myocytes
- 11:20Conclusion
En este trabajo se describe la forma típica de aislar y cultivar el corazón de rata adulta miocitos. Colagenasa y la proteasa se utilizan para digerir y aislar a los miocitos individuales. Miocitos cultivadas seguir este protocolo cumplir con los requisitos de la mayoría de los experimentos.
Tags
Primary CultureAdult Rat Heart MyocytesCardiovascular ResearchViableCulturedMyocytesProtocolHigh YieldSingle MyocytesCa2+ ConditionsCa2+-tolerant CellsExtracellular Ca2+ ConcentrationWash StepsFilteredResuspendedCulture MediumLaminin Coated SlipsExperimentsElectrophysiologyBiochemistryImagingMolecular Biology
