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蛋白质组学鉴定蛋白质,P2X2配体门控的阳离子通道交互
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JoVE Journal
Biology
Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels
Please note that all translations are automatically generated.
Click here for the English version.
蛋白质组学鉴定蛋白质,P2X2配体门控的阳离子通道交互
DOI:
10.3791/1178-v
•
16:36 min
•
May 18, 2009
•
Harpreet Singh
,
Sarah Warburton
,
Thomas M. Vondriska
,
Baljit S. Khakh
1
Department of Physiology
,
David Geffen School of Medicine, University of California, Los Angeles
,
2
Department of Anesthesiology
,
David Geffen School of Medicine, University of California, Los Angeles
,
3
Department of Anesthesiology, Medicine and Physiology
,
David Geffen School of Medicine, University of California, Los Angeles
Chapters
00:00
Title
01:01
Introduction
01:35
Subcloning, Expressing, and Binding a GST-Fusion protein to GLU-coated beads
06:41
The GST-fusion Protein Binding Assay
09:12
Preparing gel-separated proteins for Mass Spectrometry
15:33
Representative Results/ Outcome
16:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述一个简单的协议,以确定大脑的蛋白质结合ATP门控P2X2受体的C末端全长。这种方法的延伸和系统应用到所有P2X受体预计将导致一个更好地了解P2X受体信号。
Tags
Proteomics
Proteins
P2X2 Ligand-gated Cation Channels
Ligand-gated Ion Channels
Synaptic Communication
Nervous System
Cys Loop
Glutamate-gated Channels
P2X Receptor Channels
Transmitter Binding
Pore Opening
Ions Flow
Electrochemical Gradients
Calcium Ions
Downstream Signaling Roles
Gene Regulation
Protein Signaling Complexes
C-terminal Aspects
P2X2 Receptor Cytosolic Domain
ATP-gated Cation Channels
Subunits
Excitatory Synaptic Transmission
Neurotransmitter Release
Excitable Cells
Non-excitable Cells
Neuronal Signaling
Inflammation
Cardiovascular Function
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