A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 00:09Introduction
- 00:39cDNA synthesis from RNA
- 02:52Double-Stranded cDNA clean-up
- 05:55in vitro Transcription
- 06:31aRNA Clean-up After IVT
- 09:41Conclusion
Tags
Vaccinia Virus InfectionTemporal AnalysisVirus Gene ExpressionPoxviridae FamilyOrthopox GenusVariolaSmallpoxMonkeypoxVaccinia (VAC)Viral GenomeEarly GenesIntermediate GenesLate GenesRNA PolymerasesHost Cellular EnvironmentTranscript AbundanceTime Course InfectionsHeLa CellsRNA IsolationMicroarraysGene Expression Analysis
