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自制的网站定向诱变整个质粒
JoVE Journal
Biology
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JoVE Journal
Biology
Homemade Site Directed Mutagenesis of Whole Plasmids
Please note that all translations are automatically generated.
Click here for the English version.
自制的网站定向诱变整个质粒
DOI:
10.3791/1135-v
•
07:11 min
•
May 11, 2009
•
Mark Laible
,
Kajohn Boonrod
1
Department of Biology
,
Johannes Gutenberg-University Mainz, Germany
,
2
Proteomics division, AlPlanta
,
Neustadt an der Weinstrasse, Germany
Chapters
00:05
Title
00:10
Introduction
00:47
What We Need
02:47
Thermocycling Reaction
03:29
Thermocycling Conditions
04:09
Gel Check After Reaction
04:28
DpnI Digestion and Transformation
04:58
Transformation
05:30
Screening of Clones
06:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
网站定向诱变整个质粒是一种简单的方法来创建原始质粒略有不同的变化。在这里,我们展示了一个简单而成本效益的方法,引入碱基质粒使用的标准试剂。
Tags
Site Directed Mutagenesis
Whole Plasmids
Cloned Target Gene
Substitution
Deletion
Insertion
Amplifying Plasmid
Mutagenic Primers
Base Substitutions
Protocol
Cost-effective
Commercial Kits
Reaction Cost Reduction
Critical Steps
Mutagenic Primer Design
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