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植物激素在应激反应监测
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Biology
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JoVE Journal
Biology
Monitoring Plant Hormones During Stress Responses
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植物激素在应激反应监测
DOI:
10.3791/1127-v
•
11:01 min
•
June 15, 2009
•
Marie J. Engelberth
,
Jurgen Engelberth
1
Department of Biology
,
University of Texas
Chapters
00:05
Title
00:39
Introduction
01:10
Extraction of Plant Material
04:16
Methylation and Preparation of Filters
06:43
Collecting Plant Extraction
08:10
Analysis of Samples
09:58
Representative Results/ Outcome
10:30
Conclusion
Summary
Automatic Translation
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提供一个简单的方法是,允许小组织样本的多种植物激素的快速提取和分析。该程序使用气相萃取庄严的纯化步骤。 GC / MS化学电离主要生产(M +1)+离子的样品进行了分析。
Tags
Plant Hormones
Stress Responses
Environmental Stimuli
Insect Herbivory
Pathogen Infection
Drought Stress
Jasmonic Acid (JA)
Salicylic Acid (SA)
Abscisic Acid (ABA)
Temporal Monitoring
Spatial Monitoring
Vapor Phase Extraction
Gas Chromatography/mass Spectrometry (GC/MS) Analysis
Quantification
Internal Standards
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