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Chapters
- 00:00Title
- 00:58Introduction
- 01:27Disruption of the Influenza A Virion
- 02:53Glycerol Gradient Sediment Velocity Centrifugation
- 04:18Analysis of the Glycerol Gradient Fractions by SDS Polyacrylamide Gel Electrophoresis
- 05:40Concentration of the vRNP Fractions
- 06:39Negative Staining of vRNPs
- 08:25Representative Results/ Outcome
- 09:05Conclusion
El genoma del virus de influenza A se compone de ocho complejos separados de ARN y proteínas, denominadas complejos de ribonucleoproteína viral (vRNPs). Este trabajo describe la purificación gradiente de glicerol y de microscopía electrónica de transmisión de visualización de la gripe A vRNPs.
Tags
PurificationVisualizationInfluenza AViral Ribonucleoprotein ComplexesVRNPsViral EnvelopeNuclear ImportReplicationIsolated VRNPsProcedureDisruptionDetergentsGlycerol GradientVelocity SedimentationSDS-PAGECoomassie Blue StainingPeak FractionsConcentrationCentrifugationIntegrity VerificationTransmission Electron Microscopy
