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Biology
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可行的小鼠IL - 17分泌性T细胞的检测和隔离
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
Please note that all translations are automatically generated.
Click here for the English version.
可行的小鼠IL - 17分泌性T细胞的检测和隔离
DOI:
10.3791/1037-v
•
12:38 min
•
December 18, 2008
•
Anna Foerster
,
Mario Assenmacher
,
Michaela Niemoeller
,
Elly Rankin
,
Mariette Mohaupt
,
Anne Richter
1
Miltenyi Biotec,GmbH
Chapters
00:00
Title
01:30
Introduction
02:10
Preparing Reagents
03:07
Stimulating the Splenocytes
05:19
Labelling Cells with the Catch Reagent
07:36
Labeling Cells with IL-17 Detection Antibody (Biotin) and Anti-Biotin-PE
09:50
FACS Analysis
11:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
此过程描述了小鼠Th17细胞的白细胞,积极分泌刺激后,IL - 17的检测和隔离。
Tags
Detection
Isolation
Viable
Mouse
IL-17-secreting T Cells
MACS Cytokine Secretion Assay
Single Cell Level
Sensitive Isolation
Cytokine-secreting Cells
IL-17-secreting Cells
Single Cell Suspension
Mouse Splenocytes
PMA/ionomycin
Cytokine Secretion
IL-17 Catch Reagent
Bi-specific Antibody
CD45
Leukocytes
IL-17
Secretion Restart
Temperature Increase
Trapped IL-17
Flow Cytometry
Anti-Biotin-PE Antibody
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