A non-HEPES proprietary medium suitable for supporting cell growth for a variety of epithelial, fibroblast, and lymphoid cell lines in atmospheric conditions, contains no L-glutamine
Trypsin-EDTA
Reagent
Invitrogen
25200-072
PBS
Reagent
Invitrogen
14190-144
Phosphate-buffered saline, without calcium or magnesium
Pen/Strep
Reagent
Invitrogen
15140-122
Contains 10,000 units of penicillin (base) and 10,000 g of streptomycin (base)/ml utilizing penicillin G (sodium salt) and streptomycin sulfate in 0.85% saline
FBS
Reagent
Invitrogen
10437-028
Fetal Bovine Serum, Qualified
Horse Serum
Reagent
Invitrogen
16050-130
Horse Serum, EIA tested (negative) serum
Fibronectin
Reagent
Sigma-Aldrich
F0895
Fibronectin from human plasma, also called cold insoluble globulin
EGF
Reagent
Sigma-Aldrich
E4127
Epidermal Growth Factor from murine submaxillary gland, lyophilized
Wong, K., Ayuso-Sacido, A., Ahyow, P., Darling, A., Boockvar, J. A., Wu, M. Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device. J. Vis. Exp. (12), e674, doi:10.3791/674 (2008).